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KMID : 0950619950040010067
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Journanl of Allied Health Sciences
1995 Volume.4 No. 1 p.67 ~ p.74
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Detection of Methicillin-Resistant S. aureus(MRSA) by Polymerase Chain Reaction Amplification on the mecA Gene
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Lee Seung-Gwan
Lee Chang-Kyu
Whang Sun-Chul
Lee Do-Hyun
Lee Dong-Hoo
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Abstract
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Polymerase chian reaction(PCR) was used for amplification of the methicillin-resistant Staphylococcus aureus(M´RSA) mecA gene, encoding the penicillin-binding protein 2a(PBP 2a) which is associated with staphylococcal methicillin resistance. A total of 80 strains of staphylococcal strains were tested by means of PCR, and the data obtained were compared with in vitro oxacillin susceptibility antibiograms. The strains were independently diagnosed as oxacillin resistant S. aureus and classified as MRSA based on the NCCLS antibiotics susceptibility test interpretation. The PCR products were further verified by agarose gel electrophoresis to exhibit amplified DNA fragments. Of 80 oxacillin resistant S. aureus tested, mecA-positive strains. were 77 while 3 strains tested showed mecA-negative.
Agreement of MRSA determination between PCR and agar diffusion susceptibility test was 96%. 13 strains of oxacillin-susceptible S. aureus were projected for PCR as controls and found that all the strains (100%) were wecA-negative. None of the 13 strains showed mecA-positive.
It was proved that PCR is very reliable genomic method for rapid, sensitive and accurate PBP 2a gene detection of methicillin-resistant S. aureus.
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